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> Stages de M2 > Liste des stages proposés pour l’année 2015-2016 > Addressing the biological function of nucleus centration in mammalian (...)

Addressing the biological function of nucleus centration in mammalian oocytes.

proposé par Marie-Hélene VERLHAC, CIRB, Collège de France, 11 place Marcelin Berthelot, 75005 Paris

Projet de stage :

Nucleus positioning is involved in many functions such as cell differentiation during development and errors in nucleus positioning can lead to diseases. A correlation between central nucleus location and success of meiotic divisions has been established in mouse and human oocytes. Yet, the exact biological function of nucleus centration in mammalian oocytes remains a terra incognita. This is even more surprising considering that oocytes have to undergo extremely asymmetric meiotic divisions afterwards and to achieve this, they need to position their chromosomes away from the centre in the ensuing divisions (Verlhac 2000). Oocytes invalidated for the actin nucleator, Formin 2, present off-centred nuclei (Dumont 2007 ; Azoury 2008). The re-introduction of Formin 2 in these oocytes allows efficient nucleus centration (Almonacid, 2015). By performing a two-hybrid screen using the N-terminus of Formin 2 as a bait on a mouse ovary cDNA library, we have isolated two sub-units belonging to the same transcriptional repressive complex. In this project, we will study the role of one of these two Formin 2 partner in the process of maternal transcriptional silencing at the end of oocyte growth.

Techniques mises en œuvre par le stagiaire :

oocyte collection and culture, in vivo imaging using spinning disk confocal microscopy, Super-resolution microscopy, 3D-reconstruction, Quantitative microscopy.

Documents joints

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