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Chip analysis of genetic and epigenetic modifications associated with male infertility and reduced sperm counts

proposé par Ken Mc ElReavey Laboratoire de Reproduction, Fertilité et Populations, Institut Pasteur, Rue du Dr Roux – 75015 Paris

Genetic (or epigenetic) causes of male infertility are poorly understood. Cytogenetically detectable chromosomal rearrangements, Y chromosome microdeletions and mutations in genes involved in spermatogenesis account for spermatogenic failure in a small percentage of cases. There is also considerable evidence for a decline in sperm counts in some European countries suggesting that environmental factors may be acting. The project consists of two parallel genetic and epigenetic approaches to understand male spermatogenic failure associated global decline in sperm counts. The former consists of applying ultra-high resolution oligoarray comparative genomic hybridization to detect gene copy number changes at a whole genome level (resolution 50 Kb) in azoospermic men. The epigenetic analyses consists of measuring global changes of methylation status at 35,000 CpG islands in sperm from men with reduced sperm counts or normospermic men using 100K mapping arrays from affymetrix.

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