> Stages de M2 > Liste des stages proposés pour l’année 2007-2008 > Cholinergic receptors in the human oviduct

Cholinergic receptors in the human oviduct

proposé par Wolfgang Kummer, Institute for Anatomy and Cell Biology, Justus-Liebig-University, Aulweg 123, D-35385 Giessen, Germany

Projet de stage : In clinical practice, it has been noticed that smoking is a major risk factor for extra-uterine gravidity. The reason for this is not clear. Nicotine is a major compound in cigarette smoke and may be responsible for it. In the body, nicotine acts on acetylcholine receptors of the nicotinic type. Fifteen different subunits are known that can group together to build up a full nicotinic receptor. There is also another type of acetylcholine receptors, named muscarinic receptors – here five different receptors are known. The aim of the project will be to identify those cells in the human uterine tube that express the different nicotinic and muscarinic acetylcholine receptor types. This can be done on tissue sections by using receptor-specific antibodies (immunohistochemistry), specificity will be validated by Western blotting.

Techniques utilisées : Single- and double-labelling immunohistochemistry, fluorescence microscopy, confocal laser-scanning microscopy, Western blotting

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