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> Stages de M2 > Liste des stages proposés pour l’année 2013-2014 > Cytoskeleton reorganization during cell-cell fusion process and tumor (...)

Cytoskeleton reorganization during cell-cell fusion process and tumor progression

proposé par Guillaume PIDOUX, INSERM Paris Descartes

Résumé du thème de recherche de l’équipe :

The group’s work addresses a complex biological phenomenon : the cell fusion, which occurs in fertilization, fetal development, skeletal muscle formation, bone homeostasis and tumor progression. Cell fusion and syncytial formation result in the mixing of plasma membrane components and merging of cell content between two or more cells. Currently, the "cell fusion" group is focused on understanding spatiotemporal regulation of intracellular signaling pathways and in particular, how cAMP signaling networks cross-talk with and integrate into the complex web of signaling pathways. In order to perform their research, the group also establishes new tools and technology platforms for in-depth study of these pathways and to promote the development of highly specific pharmaceuticals with minimal side-effects. The ultimate aim is to understand the molecular mechanisms governing cell fusion processes in models such as trophoblasts and cancer cells.

Projet de stage :

Cell fusion processes are complex biological phenomena essential in fertilization, fetal development, skeletal muscle formation and bone homeostasis. Moreover, cell fusion has recently been shown to play a role in the metastatic process. Cell fusion and syncytial formation result in the mixing of plasma membrane components and merging of cell content between two or more cells. Although occurring in different biological contexts, cell fusion processes share many of the same steps. A cytoskeleton reorganization is needed to commit cell fusion. However, the precise role of cytoskeleton in cell fusion remains mysterious. Specifically, how could organization of the intracellular cytoskeleton and its spatiotemporal regulation affect plasma membrane dynamics and fusion. Despite the paucity of studies on cell fusion in human cancer, accumulating experimental evidence in recent years has suggested a possible broad involvement of cell fusion during the initiation, progression, and phenotypic diversification of cancer. By different approaches of live cell imaging, fusion assays, immune-cytology and pharmacokinetics, we propose to study the spatiotemporal regulation of the cAMP signaling on the cytoskeleton reorganization prior to cell fusion in cancer cells

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