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> Stages de M2 > Liste des stages proposés pour l’année 2015-2016 > Genetics and Pathophysiology of human asthenozoospermia.

Genetics and Pathophysiology of human asthenozoospermia.

proposé par Aminata TOURE, Inserm U1016, Génomique, Epigénétique et Physiopathologie de la Reproduction, Institut Cochin, 24 rue du Fbg Saint Jacques, 75014 Paris

Projet de stage :

Infertility is a major public health issue currently affecting an estimated 7%–12% of couples worldwide. Various sperm defects, including asthenozoospermia, which is defined by the absence or the reduction of sperm motility are responsible for male infertility. Asthenozoospermia is detected in more than 40% of infertile men and is often associated with lower levels of sperm production and morphological abnormalities (oligo-astheno-teratozoospermia). Despite its high prevalence, very little is known about the genetic abnormalities potentially underlying human asthenozoospermia. During the last years our laboratory has been interested in the molecular mechanisms underlying the process of sperm motility and capacitation together with the pathophysiological events associated with asthenozoospermia. In particular, we have identified the sperm specific protein SLC26A8 as an anion exchanger essential for the activation of the sperm motility ; our work indicates SLC26A8 cooperates with the chloride channel CFTR to control sperm motility in both mouse and human. The internship we propose aims at identifying further genes required for the control of sperm motility by a genetic approach and characterizing the molecular mechanisms that are implicated. The program involves a broad range of technics in the field of genetics and cellular biology : molecular biology, biochemistry, cell culture, immunohistochemistry, electron microscopy.

Techniques mises en œuvre par le stagiaire :

Biologie moléculaire, biochimie, culture cellulaire, Immuno-histochimie, microscopie photonique, confocale et electronique.

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