> Stages de M1 > Stages proposés pour l’année 2012-2013. > Génétique de la spermatogenèse

Génétique de la spermatogenèse

proposé par Stéphane Viville, INSERM-CNRS, Strasbourg

Projet de stage : We recently characterized two new mammalian-specific genes, Tex19.1 and its paralog Tex19.2. Both genes are expressed in pachytene spermatocytes in adult testes. In addition, Tex19.1 is expressed in pluripotent cells (ES, EG, iPS and PGC cells), the inner cell mass of blastocysts and the placenta. In order to decipher Tex19 functions, we generated three types of knockout (KO) : i) KO of Tex19.1 ii) KO of Tex19.2 iii) double KO (DKO) of both genes. The major phenotype observed is male infertility with an up-regulation of one endogenous retrovirus, MMERVK10C. Furthermore, GST pulldown experiments showed that proteins from the piRNA pathway are potential candidate partners of Tex19. So in order to validate Tex19 partners, we will perform IP and western blot experiments based on Co-transfections in eukaryotic cells of vectors expressing flagged Tex19 protein and vectors expressing all tex19 tagged partners.

Techniques mises en œuvre par le stagiaire : Cloning, cell culture, transfection, IP, westernblot.

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