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> Stages de M2 > Liste des stages proposés pour l’année 2016-2017 > Links between the histone H3K4 methyltransferase Set1 complex and meiotic (...)

Links between the histone H3K4 methyltransferase Set1 complex and meiotic recombination 

proposé par Valérie BORDE, UMR3664 CNRS Institut Curie 75005 Paris

Projet de stage : In meiosis, programmed DSBs are formed by the Spo11 protein, and their repair by homologous recombination creates the crossovers, necessary for the correct segregation of homologs during the first meiotic division. We have shown in the lab that a histone modification, histone H3K4 methylation, is important for the formation of meiotic DSBs and their correct localization (Borde et al, 2009, EMBO J). This histone modification was later shown to be important for meiotic recombination in Mammals. Recently, we uncovered the molecular mechanism linking this histone modification to the formation of meiotic DSBs. We found that a protein called Spp1 contains a PHD finger module that specifically interacts with histone H3K4me3 and interacts with the proteins that make meiotic DSBs (Sommermeyer et al, 2013, Molecular Cell). Spp1 is thus required for the normal position and frequency of meiotic DSBs. However, residual DSB and recombination occurs in the absence of Spp1, suggesting alternate or compensating mechanisms. The M2 project will aim at characterizing the pathways of meiotic recombination used in the absence of Spp1. The M2 student will test the function of several candidates identified in a recent genetic screen aimed at identifying the factors that promote recombination in the absence of Spp1.

Techniques mises en œuvre par le stagiaire : The approaches will use genome-wide ChIPseq experiments, genetic and biochemical approaches.

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