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> Stages de M2 > Liste des stages proposés pour l’année 2020-2021 > Microglia responses in genetic epilepsy models

Microglia responses in genetic epilepsy models

proposé par Nadia Soussi-yanicostas, Université de Paris, NeuroDiderot, Inserm, F-75019 Paris, France

Projet de stage : In order to better understand the role of microglial cells in epilepsy, our team takes advantage of the large set of in vivo approaches allowed by the zebrafish (Danio rerio). Specifically, we combine genetic techniques (epileptic mutant lines and transient gene knock-down) with imaging (confocal microscopy and calcium imaging), electrophysiological (EEG) and molecular approaches, to precisely characterize the phenotypic and molecular changes of microglial cells, which are induced by epileptix seizures, and better understand the consequences of these changes on subsequent brain functioning and neuron excitation

Techniques mises en œuvre par le stagiaire :
- Inactivation des gènes par morpholino
- Microinjection des embryons
- PCR quantitatives
- Immunocytochimie
- Comportement des embryons

Documents joints

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