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> Stages de M1 > Stages proposés pour l’année 2014-2015 > Molecular determinants of liver cancer disease development

Molecular determinants of liver cancer disease development

proposé par Ganna Panasyuk , Inserm U1151/CNRS UMR 8253, Necker Enfants Malades Institute (INEM), Faculté de Médecine Paris Descartes, 75 Paris

Projet de stage :

Liver cancer is the 3rd cause of death in cancer patients due to late diagnosis and lack of efficient treatment. Alarmingly, its incidence is increasing worldwide in the face of the epidemics of obesity and diabetes which are independent risk factors for liver cancer. There is an urge to understand molecular mechanisms underlying this disease. Importantly, men are four times more likely to develop liver cancer compared to women. Although this gender difference is known for a long time, its molecular determinants are not fully elucidated. Indeed, if uncovered, those could provide the avenues for development of novel therapies. Work of our laboratory has contributed to the understanding of the mechanisms of liver cancer development. Using mouse model of liver cancer driven by activated hepatic insulin signalling (hepatocyte-specific PTEN knockout mice) we discovered that in male mice metabolic reprogramming is sufficient to drive liver tumorigenesis. During the training student will contribute to thorough characterisation of female PTEN knockout mice collected at tumoral age.

Techniques mises en œuvre par le stagiaire :

During the training student will be exposed to numerous techniques including experimenting with model animals (transgenic and knockout mice), protein extraction and western blot, RNA preparation and quantitative RT-PCR, immunohistochemistry and morphometric analysis. The host laboratory is a part of a newly formed multidisciplinary Institute (http://www.institut-necker-enfants-malades.fr/). The successful candidate will be trained in highly dynamic stimulating international French-English bilingual environment. Altogether, the proposed M1 training will provide not only wet-lab initiation but will be an excellent opportunity for the student to advance his/her communication and presentation skills.

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