> Stages de M2 > stages proposés pour l’année 2019-2020 > WNT signalling and germ cell differentiation in mice

WNT signalling and germ cell differentiation in mice

proposé par Anne-Amandine CHASSOT , Institut de Biologie Valrose (iBV) CNRS UMR 7277 / INSERM UMR 1091 / UNS 06108 Nice cedex 2

Projet de stage :

Germ cells are pluripotent cells and the only ones able to give all cell populations of the individual. Hence, they can become haploid to give rise to the gametes but for this, the mitotic program has to be switched off and the meiotic program to be activated by a mechanism that still remains unknown. In the lab, we decipher how germ cells are specified and how the somatic cells instruct them to ultimately develop into functional gametes. During early embryogenesis, the primordial germ cells (PGC) proliferate, migrate and invade the developing gonads (ovaries or testes). Once in the gonad, PGC differentiate according to the sex of the embryo and become competent for meiosis. We have shown that germ cell differentiation and meiosis commitment depends on WNT/β-catenin signalling and not only on retinoic acid (RA) signalling as previous described (Chassot et al, 2011 ; Chassot et al, 2017 ; Le Rolle et al, in preparation, Chassot et al, in preparation). Now we want to deepen our understanding of the role of WNT and RA signallings on germ cell differentiation and explore meiosis initiation by mouse phenotyping using different mouse models mutant for WNT and RA signalling already available in the lab (Ctnnb1 mutants, Aldh1a mutants, RARa,b,g mutants). This project is an essential step to unravel the molecular basis of meiosis commitment and to understand such an important mechanism that is required for the survival of most of the species.

Techniques mises en œuvre par le stagiaire : The analyses will required histology (H&E, PAS staining), molecular and cellular biology methods (RT-qPCR, immunofluorescence, flow cytometry, in situ hybridization). The candidate will have a solid basis in molecular biology, cell biology and will be motivated to work on the mouse animal model.

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